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This plate above shows typical inhibition zones for traditional Kirby-Bauer antibiotic disks and an Etest gradient strip. The Minimum Inhibitory Concentration for the test antibiotic is read at the potion where the zone of inhibition intersects the Etest strip (black arrow). for Kirby-Bauer test disks, the diameter of the inhibition zone is measured; the diameter of the zone must be equal to or greater than the value given in the lab manual for the test organism to be assessed as sensitive to the antibiotic.The image below shows coloration of the medium from growth of Pseudomonas. This organism releases a green pigment into the medium. Note that this particular strain seems rather resistant to all of the tested antibiotics. |
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