As viewed under
oil immersion; note presence
of green endospores stained with malachite green and vegetative cells
stained with safranin. Do
not mistake green ‘stuff’ that may be present on the slide after
staining for endospores -- endospores are symmetrical in shape and
appearance, and smaller than the vegetative cells (several are indicated
with black arrows). In this culture, few endospores remain within cells (an example
of an endospore in a cell is shown by the blue arrow) – essentially all
have been released.
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